Native Hydrophobic Interaction Chromatography Hyphenated to Mass Spectrometry for Characterization of Monoclonal Antibody Minor Variants
نویسندگان
چکیده
منابع مشابه
Online Hydrophobic Interaction Chromatography-Mass Spectrometry for Top-Down Proteomics.
Recent progress in top-down proteomics has led to a demand for mass spectrometry (MS)-compatible chromatography techniques to separate intact proteins using volatile mobile phases. Conventional hydrophobic interaction chromatography (HIC) provides high-resolution separation of proteins under nondenaturing conditions but requires high concentrations of nonvolatile salts. Herein, we introduce a s...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2019
ISSN: 0003-2700,1520-6882
DOI: 10.1021/acs.analchem.9b04467